Figure 1.

Yeast Two-Hybrid Interactions of RGLG2.

(A) Domain structure of RGLG2 and abbreviations of RGLG2 parts used in (B). The red dot at the N terminus indicates that amino acid Gly at position 2 is a potential site of myristoylation. Copine indicates the copine (von Willebrand factor type A) domain, and RING indicates the RING domain.

(B) Yeast cells expressed various parts of RGLG2 in the activation domain vector pGAD424 and various UBC fold proteins fused to a GAL4 DNA binding domain. pGBKT7 is the empty vector, which was used for the expression of DNA binding domain fusions to At UBC2 (a homolog of yeast Rad6/Ubc2), At UBC9 (a homolog of At UBC8 and of yeast Ubc4), or At UBC35 (a homolog of yeast Ubc13) and of MMZ2 and MMZ3 (both are homologs of yeast Mms2). The medium contains no His but 5 mM 3-aminotriazole. Only At UBC35 and the DR domains of RGLG2 show interaction in the assay. V, no insert control of vector pGAD424; S, C, D, and R, parts of RGLG2 as indicated in (A); SC, CD, DR, and CDR, regions contained in vector pGAD424. FL indicates that the complete RGLG2 protein was contained in the vector.