Figure 4.

Verification of Emission Signals from Specific Tetrapyrroles Using Mutants Grown on Norflurazon.

(A) to (L) Emission using confocal laser scanning microscopy from gun5 ([A] to [D]), crd ([E] to [H]), and wild-type ([I] to [L]) norflurazon-grown and ALA-fed seedlings. Emissions from cotyledons are shown and representative images were taken at 585 to 615 nm, 627 to 657 nm, and 680 to 710 nm for the specific emission of Mg-ProtoIX ([B], [F], and [J]), ProtoIX ([C], [G], and [K]), and chlorophyll ([D], [H], and [L]), respectively. Bars = 50μm.

(M) to (O) Corresponding fluorescence emission spectra are shown from the plastids (solid line) and cytosol (dotted line). Emission spectra were normalized to the maximum value for each measurement, and overall spectrum was calculated by averaging measurements of 10 positions overlapping (solid line) and excluding (dotted line) chloroplasts.