Figure 1.

BTH- and SA-Induced Expression of WRKY and Defense-Related Genes in Rice.

(A) BTH-induced expression. Total RNA (5 μg per lane) from the third and fourth leaves of BTH- and mock-treated plants at the indicated time points after BTH application were hybridized with an RNA probe to the 3′-untranslated regions of the gene of interest, as described in Methods. The experiments were performed in two replicates with similar results.

(B) SA-induced expression. RNA samples were prepared from SA- and mock-treated rice plants at 12 and 36 h after SA application and hybridized as in (A).