Figure 6.

Gene Silencing in ise2.

(A) to (C) Effects of sgs3-11 and ise2-2 on posttranscriptional silencing of the L1 35S∷GUS transgene. The wild-type L1 seedling (A) shows silencing of GUS, but sgs3-11 (C) and ise2-2 (B) prevent silencing in cotyledons, shoots, and leaves.

(D) to (G) Assay for miRNA-mediated silencing during ise2 embryogenesis.

(D) GFP142 line (35S:ER-GFP) expressing ubiquitous ER-GFP, except in the root.

(E) Transgenic sde1 torpedo embryo carrying the GFP171.1 construct, showing provascular GFP expression.

(F) Transgenic double mutant sde1 ise2 torpedo embryos carrying the GFP171.1 construct showing similar provascular GFP expression.

(G) Schematic representation of the GFP171.1 construct.

Bars = 1 mm in (A) to (C) and 50 μm in (D) to (F).