Figure 9.

Transient Expression of CYP79B2 and CYP71A13 in N. benthamiana Results in Conversion of Trp to IAN via IAOx.

Microsomes from leaves infiltrated with p19 (thin solid line), CYP79B2 (dotted line), or CYP79B2 and CYP71A13 (thick solid line) in combination with p19 were incubated with Trp. After incubation, the reaction mixtures were extracted with ethyl acetate, and the organic phases were analyzed by LC-MS. m/z, mass-to-charge ratio.

(A) Reconstructed ion chromatogram of m/z 175 (IAOx) from the different microsomal assays.

(B) Reconstructed ion chromatogram of m/z 155 (IAN) from the different microsomal assays.