Figure 4.

Expression Analysis of WSEP and WLHS1 Homoeologous Genes by Real-Time PCR.

(A) and (B) Real-time PCR was performed using homoeolog-specific primers for WSEP-A, WSEP-B, and WSEP-D (A) and for WLHS1-A, WLHS1-B, and WLHS1-D (B). ACTIN was used as endogenous control. Total RNAs were isolated from spikes of CS plants at various developmental stages and from various floral organs at the booting stage.

(C) Transcript levels of the WLHS1 genes in young spikes (10 to 25 mm in length) of diploid, tetraploid, and hexaploid species and in immature spikes (booting stage) of a synthetic hexaploid. The genome constitution of each species is indicated in parenthesis. Note that the silencing of WLHS1-B was observed only in hexaploid CS wheat (T. aestivum). ACTIN was used as endogenous control.