Table 2.
Two- and Three-Hybrid Interactions of MADS Box Proteins of Wheat in the Yeast GAL4 System
| AD\BD | WAP1 | WAP3/WPI-1 | WAP3/WPI-2 | WAG-1 | WAG-2 | WLHS1-A | WLHS1-B | WLHS1-D | WSEP-A | WSEP-B | WSEP-D |
|---|---|---|---|---|---|---|---|---|---|---|---|
| WSEP-A | + | − | + | + | + | − | + | + | ++ | ++ | ++ |
| WSEP-B | + | − | + | + | + | − | + | + | ++ | ++ | ++ |
| WSEP-D | + | − | + | + | + | − | + | + | ++ | ++ | ++ |
| WLHS1-A | − | − | − | − | − | − | − | − | − | − | − |
| WLHS1-B | − | − | + | − | − | − | + | + | ++ | ++ | ++ |
| WLHS1-D | − | − | + | − | − | − | + | + | ++ | ++ | ++ |
Protein interactions are assessed by the viability of yeast transformants on selective medium. Relative levels of protein interaction denoted by the following: ++, strong; +, moderate; −, not detectable. AD indicates a chimeric protein with the transcriptional activation domain, and BD indicates a chimeric protein with the DNA binding domain.