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. 2007 Jun;19(6):1838–1850. doi: 10.1105/tpc.107.051599

Figure 1.

Figure 1.

Genomic Structure of T-DNA Insertion Alleles.

(A) Structure of the MDR4 gene. Boxes represent exons, and lines represent introns. The positions of the T-DNA insertions in mdr4-1 (SALK_010207) and mdr4-2 (SALK_072038) are represented by triangles.

(B) RT-PCR analysis of MDR4 transcript levels in Col-0 and the two independent mdr4 T-DNA alleles used in this study.

(C) Gene diagram of mdr1 T-DNA insertion alleles used in this study. Ws is the genetic background of the previously described mdr1-1 and mdr1-2 alleles. To obtain an allele in the Col-0 background, mdr1-3 (Salk_033455) was isolated.

(D) PCR results showing interruption of MDR1 and MDR4 in the mdr1-3 mdr4-1 double mutant (i to iv) and proper function of the gene-specific primers on wild-type DNA (v and vi). (i) MDR1 5′ primer plus MDR1 3′ primer gave no product. (ii) MDR1 5′ primer plus T-DNA Lb1a primer gave a product with the expected size. (iii) MDR4 5′ primer plus MDR4 3′ primer gave no product. (iv) MDR4 3′ primer plus T-DNA Lb1a primer gave a product with the expected size. (v) Wild-type DNA: product of the MDR1 5′ primer plus the MDR1 3′ primer was the expected size. (vi) Wild-type DNA: product of the MDR4 5′ primer plus the MDR4 3′ primer was the expected size.