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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Apr 14;1773(6):891–902. doi: 10.1016/j.bbamcr.2007.04.004

Figure 2.

Figure 2

Myospryn and RIIα interact in a cellular environment. A, RIIα and NLS-RIIα were transfected into COS cells and immunostained using an anti-RII antibody. RIIα exhibits a cytoplasmic/perinuclear localization whereas NLS-RIIα is shuttled into the nucleus. DAPI staining indicates the location of the nuclei. Arrows demonstrate representative cells. B, COS cells transfected with Myc-Spe alone or cotransfected with NLS-RIIα and Myc-Spe. Myc-Spe alone exhibits a cytoplasmic staining pattern. Cotransfection with NLS-RIIα, Myc-Spe is shuttled into the nucleus demonstrating an in vivo association with this regulatory subunit of PKA. DAPI staining indicates the location of the nuclei. C, Myospryn precipitates PKA from COS cells. COS cells were transfected with pCDNA3-Myc or Myc-Spe. Protein extracts were immunoprecipitated using the anti-MYC antibody and immunoblotted for endogenous PKA using antibodies directed against catalytic subunit (PKA-C, BD Transduction Laboratories). Protein extracts loaded and blotted with the anti-PKA-C antibody detected the presence of similar amounts of PKA-C in both samples.