Fig. 4.

Telomeric repeats cause DNA methylation-dependent transgene silencing. (A) Clones with a silenced uninsulated transgene were incubated with TSA, nicotinamide (NAM), and/or 5-azadC, as is reported in Materials and Methods. (B) As an additional example, clone 805 is shown that is sensitive to TSA. Its behavior is representative of several independent clones, like the 861 and 862 clones. Clone 805 was treated as above, and the fluorescence mean (Upper) and the percentage of positive cells (Lower) are shown.