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. 2007 Aug 21;104(35):14044–14049. doi: 10.1073/pnas.0704999104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 5. — Histone modifications over cHS4-insulated transgenes. (A) FACS profiles of the lines with telomeric (613) and random (1001) integration, respectively. (B) Duplex PCRs were used to evaluate the relative enrichment of open chromatin marks. (C and D) ChIP assays were performed with insulated telomeric (C) and random (D) integrants. Relative enrichment of histone modification was plotted, and the average enrichment values of at least two independent immunoprecipitations are shown. Each PCR was performed at least twice, and standard error is presented.