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Protection against TPE by the cHS4 does not require USF. Stable clones with the core cHS4-insulated transgene both in random or telomeric regions were stably transfected with a mixture of five USF1-specific RNAi-expressing plasmids (13). (A) Knockdown of USF1 was addressed by Western blotting. (B) RNAi-expressing subclones were maintained for 3 weeks, and GFP expression was analyzed by FACS. Representative FACS profiles of three transfections are shown.
