Fig. 2.

Generation of animals lacking Syt4. (A) Western blot of adult head extracts from control (precise excision) and syt4^BA1 mutants. One fly-head equivalent of protein was loaded into each lane and blotted by using the α-Syt4 polyclonal antibody. Although several nonspecific bands remain and serve as loading controls, the syt4^BA1 head extract is missing the abundant band corresponding to the predicted molecular weight of Syt4. (B) Immunostaining for Syt4 at larval brains (Upper) and at muscles 6 and 7 of the third-instar larval NMJ (Lower) in control and syt4^BA1 mutants. Control and mutants were imaged by using identical confocal settings.