Fig. 2.

Dyslamination in Foxc1^hith/hith mice. (a and b) Tbr1 staining of P10 brains shows labeling of layers 2/3, 5, and 6 in control and mutant brains. The mutant brains are montages generated by merging two images of both brain hemispheres. In mutant brains Tbr1⁺ cells form heterotopic clusters in the marginal zone (blue arrows in Insets) and reveal significant disorganization of cortical layers (black arrows). (c–f) Ctip2 staining labels cells in layers 5 and 6, and ER81 labels cells in layer 5, and both show significant laminar disorganization in mutant brains (black arrows); however, note that there are no Ctip2- or ER81-stained cells in the marginal zone.