Fig. 4.
Foxc1 expression in vivo at E14.5. (a and b) In both Foxc1+/ and Foxc1hith/hith heads, ventral head structures and the meninges (MN) surrounding both the cortex and the midbrain (MB) express Foxc1 mRNA. The Foxc1 signal intensity is similar in control and mutant mice. (c and d) Foxc1 protein is expressed intensely by mesenchymal cells and meningeal cells ventral to the forebrain in Foxc1+/ tissue sections. Expression of Foxc1 is not seen in neural tissue (except in pericytes associated with blood vessels; data not shown). Foxc1 protein expression in Foxc1hith/hith tissue is similarly distributed, but the staining intensity is markedly reduced. (e and f) Foxc1+ cells are distributed throughout the primitive meninges in control mice, but in Foxc1hith/hith meninges expression of Foxc1 in these cells is less. (Scale bars: 1 mm in a and b, 100 μm in c and d, and 50 μm in e and f.)