Fig. 4.

Quantities (μg/μl of hemolymph) of four storage proteins in hemolymph of P. metricus immatures from cocoon spinning to adulthood. Storage proteins are HEX1, hexamerin 1 (a); HEX2, hexamerin 2 (b); HiGLX (c); VHDL (d). Stages are L, cocooned fifth instar larva (both predefecation and postdefecation prepupae); P1, pupa up to day 11 after cocooning; P2, pupa from day 12 after cocooning; A, adult, including preemergence adults in nest cells and adults collected on the morning after emergence. F-R, foundress reared; W-R, worker-reared; development time was equal for both categories at 19 days. Only the expression level of Hex 1 (a) differed significantly during development of F-R and W-R offspring. This difference in protein levels is supported by recent results showing a corresponding 8-fold difference in mRNA levels between W-R and F-R fifth-instar larvae (J.H.H., A. L. Toth, and T. C. Newman, unpublished data). Both protein (see Fig. 5) and mRNA sequence match to the same 220 bp cDNA fragment from a putative P. metricus hexamerin gene (Contig40833).