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. 1997 Jan 21;94(2):604–609. doi: 10.1073/pnas.94.2.604

Figure 3.

Figure 3

Specific binding of BAP-135 to the combined PH and TH domains of Btk in vitro. (A) A lysate of 293 cells expressing BAP-135 was diluted 3-fold serially and incubated with agarose beads bearing GST (lanes 1–4), or GST fused to residues 1–218 (PH/TH, lanes 5–8), 219–268 (SH3, lanes 9–12), or 269–382 (SH2, lanes 13–16) of Btk. Retained protein was fractionated by SDS/PAGE and tagged BAP-135 was detected with anti-c-myc antibody 9E10. An amount of total lysate corresponding to the highest dilution used in binding assays was run in lane 17. (B) Binding assays were performed as in A, using GST fusions to residues 1–80 (PH, lanes 5–7), 104–218 (TH, lanes 8–10), or 1–218 (PH/TH, lanes 11–13) of Btk.