Figure 3.

The effect of the MHCII–hBtk transgene in Btk^+/− female mice. Cell suspensions were stained for lacZ activity, anti-IgM (biotinylated)/streptavidin-TriColor and anti-IgD-PE (spleen and MLN) or anti-CD5-PE (peritoneum). (A) The percentages of lacZ-expressing cells as mean values and standard deviations in the three peripheral B cell compartments (fraction III, IgM^highIgD^low; fraction II, IgM^high IgD^high; and fraction I, IgM^lowIgD^high; refs. 14 and 15). Values are corrected for individual variations in X chromosome inactivation ratios, using lacZ expression values from peritoneal macrophages (13). Data for spleen and MLN are from 6-week-old mice, and for peritoneum they are from 4-month-old mice. (B) Surface expression of IgM and IgD in gated lacZ⁻ and lacZ⁺ spleen cells from nontransgenic and MHCII–hBtk transgenic Btk^+/− female mice. (Upper) For comparison the profiles in (lacZ⁻) Btk⁺ males and in (lacZ⁺) Btk⁻ males are given. The distribution of IgM⁺IgD⁺ cells over the compartments III, II, and I in percentages is indicated. Data are shown as 5% probability contour plots representative of the mice examined.