Figure 4.

Correction of serum Ig levels and in vivo antigen responses by expression of the MHCII–hBtk transgene. (A) Serum concentrations of Ig subclasses in unimmunized 4-month-old mice. (B) Serum concentrations of DNP/TNP-specific antibodies were determined after primary and secondary immunizations with the TD antigen TNP-KLH, and after immunization with the TI-II antigen DNP-Ficoll in 4- to 6-month-old mice. In transgenic Btk⁺/Y males TD responses were not determined. ○, nontransgenic Btk⁺/Y males; •, MHCII–hBtk transgenic Btk⁺/Y males; □, nontransgenic Btk⁻/Y males; ▪, MHCII–hBtk transgenic Btk⁻/Y males.