Table 1.
Flow cytometric analysis of the B lymphocyte compartment in MHCII–hBtk transgenic mice
| Tissue | Cell population | Btk+/Y | Btk+/Y MHCII-hBtk | Btk−/Y | Btk−/Y MHCII–hBtk |
|---|---|---|---|---|---|
| Spleen | Nucleated cells, ×106 | 142 ± 45 | 138 ± 43 | 96 ± 30 | 113 ± 34 |
| B220+ cells, % | 59 ± 3 | 54 ± 4 | 45 ± 6* | 52 ± 9† | |
| IgMlowIgDhigh cells, % | 30 ± 4 | 26 ± 4 | 9 ± 5* | 20 ± 7† | |
| CD4+ cells, % | 18 ± 2 | 21 ± 3 | 22 ± 4 | 23 ± 3 | |
| CD8+ cells, % | 9 ± 2 | 8 ± 1 | 11 ± 2 | 11 ± 2 | |
| MLN | B220+ cells, % | 38 ± 10 | 33 ± 6 | 20 ± 5* | 31 ± 14† |
| IgMlowIgDhigh cells, % | 31 ± 8 | 22 ± 7 | 6 ± 1* | 20 ± 10† | |
| CD4+ T cells, % | 36 ± 6 | 40 ± 9 | 48 ± 7 | 40 ± 8 | |
| CD8+ T cells, % | 19 ± 4 | 20 ± 6 | 28 ± 4 | 22 ± 3 | |
| Peritoneum | CD5+ B cells, % | 4 ± 2 | 6 ± 4 | 0.3 ± 0.3* | 4 ± 2† |
| CD5− B cells, % | 13 ± 4 | 11 ± 5 | 5 ± 3* | 14 ± 5† | |
| CD5+ T cells, % | 5 ± 3 | 4 ± 2 | 7 ± 3 | 7 ± 4 | |
| Bone Marrow | B220+ cells, % | 24 ± 1 | 22 ± 1 | 19 ± 1 | 22 ± 1 |
| IgMlowIgDhigh cells, % | 2.5 ± 0.6 | 3.8 ± 1.2 | 0.7 ± 0.2* | 3.0 ± 1.4† |
Mice were 4–6 months old. Numbers of mice analyzed are 10–14 (spleen), 3–6 (MLN), 7–10 (peritoneum), and 4 (bone marrow) for all groups.
*
P < 0.05, compared with Btk+/Y mice, in the Mann–Whitney U test.
†
Not significantly different, compared with Btk+/Y mice.