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Requirements for antigen organization for CD45RO+ and CD45RA+ Th cells. The indicated numbers of CD45RO+ and CD45RA+ Th cells were pulsed with no antigen (A) or with HIV-gag or KLH (B) and coincubated with 1 × 105 CTLs and 0.3 × 105 monocytes, coexpressing HIV-gag and KLH antigens. After 9 days, cytolytic activity was assessed as described using HIV-gag pulsed T2 target cells.
