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Role of lymphokines in Th cell-dependent induction of CTLs. Culture were set up using 105 Th, 105 CTLs, and 0.3 × 105 APCs, coexpressing KLH and MAGE-3 antigens. At indicated time points, 5 units/ml rIL-2 or 500 units/ml rIL-4 (A) or neutralizing antibodies directed against IL-2, IL-4, IL-7, IL-12, and IFN-γ at 10 μg/ml (B) were added. After 11 days, 5000 51Cr-labeled and MAGE-3 pulsed T2 target cells were added, and cytolytic activity of the cultured cells was assessed as described.
