Figure 1.

Schematic representation of the mHEL expression vector. A cDNA encoding the entire HEL sequence (129 amino acids) was joined in-frame to a genomic portion of the MHC class I H-2L^d gene encoding a connecting stalk (8 amino acids), a transmembrane region (TM; 39 amino acids), and a complete cytoplasmic domain (CY; 25 amino acids). Transcription of the fusion gene was driven by the promoter region of the human cytomegalovirus (CMV) major immediate early gene. The construct extends through the genomic termination of transcription and polyadenylylation sequences used by L^d.