Table 2.

HEL peptides presented by the mutant mHEL transfectants

mHEL Mutant	Relative yield, %		Position	Sequence													Mass, amu
																	Predicted	Observed
								48	49	50	51	52	53	—	62	63	64
D48-G-G49	14	8	48–63				D	G	G	S	T	D	—	R	W		1781.90	1781.84
		6	48–64				D	G	G	S	T	D	—	R	W	W	1968.11	1968.11
	79	41	49–63					G	G	S	T	D	—	R	W		1666.81	1666.85
		38	49–64					G	G	S	T	D	—	R	W	W	1853.02	1852.99
	3	2	50–63						G	S	T	D	—	R	W		1609.76	1609.95
		1	50–64						G	S	T	D	—	R	W	W	1795.97	1796.16
	5	3	51–63							S	T	D	—	R	W		1552.70	1552.89
		2	51–64							S	T	D	—	R	W	W	1738.92	1738.85
								46	47	48	49	50	51	—	60	61	62
No D48	12	1	46–61				N	T	G	S	T	D	—	R	W		1824.97	1826.58
		11	46–62				N	T	G	S	T	D	—	R	W	W	2011.18	2011.41
	72	11	47–61					T	G	S	T	D	—	R	W		1710.86	1710.79
		61	47–62					T	G	S	T	D	—	R	W	W	1897.08	1896.89
	10	6	48–61						G	S	T	D	—	R	W		1609.76	1609.73
		4	48–62						G	S	T	D	—	R	W	W	1795.97	1795.81
	5	2	49–61							S	T	D	—	R	W		1552.70	1553.24
		4	49–62							S	T	D	—	R	W	W	1738.92	1738.73
							46	47	48	49	50	51	52	—	61	62	63
P48	0.5	0	46–62			N	T	P	G	S	T	D	—	R	W		1922.06	—
		0.5	46–63			N	T	P	G	S	T	D	—	R	W	W	2108.30	2109.81
	99	54	47–62				T	P	G	S	T	D	—	R	W		1807.98	1808.13
		45	47–63				T	P	G	S	T	D	—	R	W	W	1994.19	1994.33
	0	0	48–62					P	G	S	T	D	—	R	W		1706.88	—
		0	48–63					P	G	S	T	D	—	R	W	W	1893.09	—
	0.5	0.5	49–62						G	S	T	D	—	R	W		1609.76	1612.23
		0	49–63						G	S	T	D	—	R	W	W	1795.97	—
							46	47	48	49	50	51	52	—	61	62	63
P47	96	19	46–62			N	P	D	G	S	T	D	—	R	W		1936.07	1937.26
		73	46–63			N	P	D	G	S	T	D	—	R	W	W	2122.28	2121.86
	0	0	47–62				P	D	G	S	T	D	—	R	W		1821.96	—
	0		47–63				P	D	G	S	T	D	—	R	W	W	2008.18	—
	0	0	48–62					D	G	S	T	D	—	R	W		1724.85	—
		0	48–63					D	G	S	T	D	—	R	W	W	1911.06	—
	4	2	49–62						G	S	T	D	—	R	W		1609.76	1610.03
		2	49–63						G	S	T	D	—	R	W	W	1795.97	1796.61
						45	46	47	48	49	50	51	52	—	61	62	63
P46	47	9	45–62		R	P	T	D	G	S	T	D	—	R	W		2079.26	2080.77
		38	45–63		R	P	T	D	G	S	T	D	—	R	W	W	2265.47	2267.37
	0	0	46–62			P	T	D	G	S	T	D	—	R	W		1923.10	—
		0	46–63			P	T	D	G	S	T	D	—	R	W	W	2109.28	—
	0	0	47–62				T	D	G	S	T	D	—	R	W		1825.95	—
		0	47–63				T	D	G	S	T	D	—	R	W	W	2012.17	—
	1	0	48–62					D	G	S	T	D	—	R	W		1706.88	—
		1	48–63					D	G	S	T	D	—	R	W	W	1893.09	1893.86
	53	9	49–62						G	S	T	D	—	R	W		1609.76	1610.81
		44	49–63						G	S	T	D	—	R	W	W	1795.97	1797.21
					44	45	46	47	48	49	50	51	52	—	61	62	63
P45	11	11	44–62	N	P	N	T	D	G	S	T	D	—	R	W		2151.28	2151.80
		0	44–63	N	P	N	T	D	G	S	T	D	—	R	W	W	2337.49	—
	0	0	45–62		P	N	T	D	G	S	T	D	—	R	W		2037.17	—
		0	45–63		P	N	T	D	G	S	T	D	—	R	W	W	2223.39	—
	0	0	46–62			N	T	D	G	S	T	D	—	R	W		1940.06	—
		0	46–63			N	T	D	G	S	T	D	—	R	W	W	2126.27	—
	21	8	47–62				T	D	G	S	T	D	—	R	W		1825.95	1826.52
		13	47–63				T	D	G	S	T	D	—	R	W	W	2012.17	2011.96
	55	31	48–62					D	G	S	T	D	—	R	W		1724.85	1724.65
		24	48–63					D	G	S	T	D	—	R	W	W	1911.06	1910.64
	12	6	49–62						G	S	T	D	—	R	W		1609.76	1609.59
		6	49–63						G	S	T	D	—	R	W	W	1795.97	1795.68
								47	48	49	50	51	52	—	61	62	63
P44	19	2	47–62				T	D	G	S	T	D	—	R	W		1825.95	1825.70
		17	47–63				T	D	G	S	T	D	—	R	W	W	2012.17	2011.30
	77	34	48–62					D	G	S	T	D	—	R	W		1724.85	1724.58
		43	48–63						G	S	T	D	—	R	W	W	1911.06	1910.47
	4	1	49–62						G	S	T	D	—	R	W		1609.76	1609.84
		3	49–63						G	S	T	D	—	R	W	W	1795.97	1796.37

Sequences of the 6 recovered HEL peptides given in single letter amino acid code. (—), a fragment that was not recovered. Amino acids changed by mutagenesis given in bold.