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. 1997 Jan 21;94(2):646–651. doi: 10.1073/pnas.94.2.646

Table 1.

Effect of soluble hIgG on the phenotype of lymphocytes from secondary lymphoid tissue

Treatment Blood
Lymph node
Spleen
Spleen (permeabilized)
% hIgM (mean fl) % B220 % hIgM (mean fl) % B220 % hIgM (mean fl) % B220 % hIgM (mean fl)
None 9.4 ± 0.7 15.1 ± 1.3 13.1 ± 0.9 16.1 ± 1.8 20.9 ± 1.5 37.9 ± 2.4 29.9 ± 4.1
(320) (680) (751) (283)
12 h 4.1 ± 0.6* 8.3 ± 0.7 14.9 ± 4.0 21.1 ± 3.5 23.0 ± 4.3 36.6 ± 3.5 33.7 ± 4.5
(105)* (138)* (200)* (381)
24 h 3.0 ± 0.5* 6.8 ± 0.7 15.4 ± 2.1 27.5 ± 5.7* 32.3 ± 3.2* 42.8 ± 1.9 39.3 ± 4.9
(121)* (177)* (279)* (551)
4 days 2.9 ± 0.6* 6.5 ± 0.5 5.1 ± 1.5* 12.2 ± 0.7 8.7 ± 1.5* 25.6 ± 3.1* 14.4 ± 3.4*
(134)* (203)* (257)* (367)
7 days 3.4 ± 1.9* 6.1 ± 2.4 4.7 ± 1.6* 9.4 ± 2.9 6.8 ± 1.8* 22.2 ± 2.4* 9.9 ± 1.2*
(232)* (283)* (365)* (276)

AB29 mice were injected i.p. with 2 mg DHGG and killed at various times after treatment. Lymphoid cells from spleen and blood were stained for expression of either B220 (total B cells) or hIgM and then analyzed on the cytofluorograph. Data are expressed as mean percentage positive cells ± SE and mean fluorescence (in parentheses) for groups of three mice. 

*

P < 0.05.