Table 1.
Effect of soluble hIgG on the phenotype of lymphocytes from secondary lymphoid tissue
Treatment | Blood
|
Lymph node
|
Spleen
|
Spleen (permeabilized)
|
|||
---|---|---|---|---|---|---|---|
% hIgM (mean fl) | % B220 | % hIgM (mean fl) | % B220 | % hIgM (mean fl) | % B220 | % hIgM (mean fl) | |
None | 9.4 ± 0.7 | 15.1 ± 1.3 | 13.1 ± 0.9 | 16.1 ± 1.8 | 20.9 ± 1.5 | 37.9 ± 2.4 | 29.9 ± 4.1 |
(320) | (680) | (751) | (283) | ||||
12 h | 4.1 ± 0.6* | 8.3 ± 0.7 | 14.9 ± 4.0 | 21.1 ± 3.5 | 23.0 ± 4.3 | 36.6 ± 3.5 | 33.7 ± 4.5 |
(105)* | (138)* | (200)* | (381) | ||||
24 h | 3.0 ± 0.5* | 6.8 ± 0.7 | 15.4 ± 2.1 | 27.5 ± 5.7* | 32.3 ± 3.2* | 42.8 ± 1.9 | 39.3 ± 4.9 |
(121)* | (177)* | (279)* | (551) | ||||
4 days | 2.9 ± 0.6* | 6.5 ± 0.5 | 5.1 ± 1.5* | 12.2 ± 0.7 | 8.7 ± 1.5* | 25.6 ± 3.1* | 14.4 ± 3.4* |
(134)* | (203)* | (257)* | (367) | ||||
7 days | 3.4 ± 1.9* | 6.1 ± 2.4 | 4.7 ± 1.6* | 9.4 ± 2.9 | 6.8 ± 1.8* | 22.2 ± 2.4* | 9.9 ± 1.2* |
(232)* | (283)* | (365)* | (276) |
AB29 mice were injected i.p. with 2 mg DHGG and killed at various times after treatment. Lymphoid cells from spleen and blood were stained for expression of either B220 (total B cells) or hIgM and then analyzed on the cytofluorograph. Data are expressed as mean percentage positive cells ± SE and mean fluorescence (in parentheses) for groups of three mice.
P < 0.05.