Table 4.
In vivo secretion of hIgM
| Serum levels of hIgM
|
No. of IgM RF-secreting B cells
|
|||
|---|---|---|---|---|
| Time after hIgG Injection, days | T cell help (bm12) | Serum hIgM, mg/ml | No./106 spleen cells | No./106 hIgM+ B cells |
| − | 12.7 ± 4.2 | 12 ± 9 | 44 ± 32 | |
| 1 | − | 12.7 ± 3.1 | 1,059 ± 657 | 4,403 ± 3567 |
| 3 | − | 11.2 ± 12.1 | 1,310 ± 1138 | 10,562 ± 8370 |
| 7 | − | 8.2 ± 1.4 | 119 ± 70 | 1,824 ± 1237 |
| 1 | + | 23.7 ± 3.1 | 10,104 ± 1986* | 36,061 ± 9999* |
| 7 | + | 201.3 ± 43.6* | 4,087 ± 3670* | 33,094 ± 32,819* |
AB29 mice were injected i.p. with 2 mg DHGG and killed at either 1, 3 or 7 days after treatment. Additional groups included mice injected i.p. with 2 mg DHGG and i.v. with 20 × 106 C57Bl/6bm12 splenocytes (T cell help) 7 days before sacrifice and mice injected i.p. with 2 mg DHGG and i.v. with 20 × 106 C57Bl/6bm12 splenocytes (T cell help) 1 and 4 days, respectively, before sacrifice. Mice were bled at sacrifice and serum human IgM levels measured by ELISA, and numbers of human IgM RF secreting splenocytes were measured by ELISPOT using fresh spleen. Data are expressed as the means ± SD of groups of three or four mice. Numbers per 106 hIgM+ cells were calculated based on numbers of permeabilized splenocytes which stained positive for hIgM.
P < 0.05.