Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1992 May;58(5):1776–1779. doi: 10.1128/aem.58.5.1776-1779.1992

Degradation of triglycerides by a pseudomonad isolated from milk: molecular analysis of a lipase-encoding gene and its expression in Escherichia coli.

L A Johnson 1, I R Beacham 1, I C MacRae 1, M L Free 1
PMCID: PMC195672  PMID: 1622251

Abstract

Psychrotrophic lipolytic bacteria represent a significant problem in the storage of refrigerated dairy products. A lipase-encoding gene has been cloned and characterized from a highly lipolytic strain of Pseudomonas. The nucleotide sequence of the gene predicts a polypeptide of M(r) 49,905, which was identified when the gene was expressed in Escherichia coli.

Full text

PDF
1776

Images in this article

1776Image
on p.1776

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson R. E., Hedlund C. B., Jonsson U. Thermal inactivation of a heat-resistant lipase produced by the psychotrophic bacterium Pseudomonas fluorescens. J Dairy Sci. 1979 Mar;62(3):361–367. doi: 10.3168/jds.s0022-0302(79)83252-x. [DOI] [PubMed] [Google Scholar]
  2. Antonian E. Recent advances in the purification, characterization and structure determination of lipases. Lipids. 1988 Dec;23(12):1101–1106. doi: 10.1007/BF02535273. [DOI] [PubMed] [Google Scholar]
  3. Benz R. Structure and function of porins from gram-negative bacteria. Annu Rev Microbiol. 1988;42:359–393. doi: 10.1146/annurev.mi.42.100188.002043. [DOI] [PubMed] [Google Scholar]
  4. Brenner S. The molecular evolution of genes and proteins: a tale of two serines. Nature. 1988 Aug 11;334(6182):528–530. doi: 10.1038/334528a0. [DOI] [PubMed] [Google Scholar]
  5. Cox J. M., Mac Rae I. C. A numerical taxonomic study of proteolytic and lipolytic psychrotrophs isolated from caprine milk. J Appl Bacteriol. 1989 Feb;66(2):137–152. doi: 10.1111/j.1365-2672.1989.tb02463.x. [DOI] [PubMed] [Google Scholar]
  6. Gold L., Pribnow D., Schneider T., Shinedling S., Singer B. S., Stormo G. Translational initiation in prokaryotes. Annu Rev Microbiol. 1981;35:365–403. doi: 10.1146/annurev.mi.35.100181.002053. [DOI] [PubMed] [Google Scholar]
  7. Hohn B., Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene. 1980 Nov;11(3-4):291–298. doi: 10.1016/0378-1119(80)90069-4. [DOI] [PubMed] [Google Scholar]
  8. Jørgensen S., Skov K. W., Diderichsen B. Cloning, sequence, and expression of a lipase gene from Pseudomonas cepacia: lipase production in heterologous hosts requires two Pseudomonas genes. J Bacteriol. 1991 Jan;173(2):559–567. doi: 10.1128/jb.173.2.559-567.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kraft R., Tardiff J., Krauter K. S., Leinwand L. A. Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase. Biotechniques. 1988 Jun;6(6):544-6, 549. [PubMed] [Google Scholar]
  10. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  11. SARDA L., DESNUELLE P. Action de la lipase pancréatique sur les esters en émulsion. Biochim Biophys Acta. 1958 Dec;30(3):513–521. doi: 10.1016/0006-3002(58)90097-0. [DOI] [PubMed] [Google Scholar]
  12. Shelley A. W., Deeth H. C., MacRae I. C. A numerical taxonomic study of psychrotrophic bacteria associated with lipolytic spoilage of raw milk. J Appl Bacteriol. 1987 Mar;62(3):197–207. doi: 10.1111/j.1365-2672.1987.tb02399.x. [DOI] [PubMed] [Google Scholar]
  13. Shelley A. W., Deeth H. C., MacRae I. C. Comparison of a simple butterfat agar medium with other media used for isolation and enumeration of lipolytic bacteria from dairy products. J Dairy Res. 1987 Aug;54(3):413–420. doi: 10.1017/s0022029900025590. [DOI] [PubMed] [Google Scholar]
  14. Shelley A. W., Deeth H. C., MacRae I. C. Growth of lipolytic psychrotrophic pseudomonads in raw and ultra-heat-treated milk. J Appl Bacteriol. 1986 Nov;61(5):395–400. doi: 10.1111/j.1365-2672.1986.tb04302.x. [DOI] [PubMed] [Google Scholar]
  15. Tabor S., Richardson C. C. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. doi: 10.1073/pnas.84.14.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vieira J., Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. doi: 10.1016/0076-6879(87)53044-0. [DOI] [PubMed] [Google Scholar]
  17. West S. E., Iglewski B. H. Codon usage in Pseudomonas aeruginosa. Nucleic Acids Res. 1988 Oct 11;16(19):9323–9335. doi: 10.1093/nar/16.19.9323. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES