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Immunohistochemistry of VEGF expression. Cytospin preparations of Dami cells and megakaryocytes were stained with Wright–Giemsa (A and B, respectively) and analyzed with immunohistochemistry for VEGF expression (C and D). The megakaryocytic cell line Dami was positive for VEGF (C), particularly with increasing cell size. Among the ex vivo-generated megakaryocytic cells, only highly polyploid, large megakaryocytes stained positive for VEGF (D). (×1000.)
