Figure 7. BDCA-1 and CD163 are alternative markers for CD11c and FXIIIA, respectively.

(A) BDCA-1 and CD163 identified discrete populations of dermal cells. (B) BDCA-1⁺ cells (red circle) and CD163⁺ cells (blue circle) were also discrete populations in dermal single-cell suspensions. (C) FACS histograms gated on BDCA-1⁺ cells (red line), CD163⁺ cells (blue line), or isotype (green line). BDCA-1⁺ cells were CD11c^hi, FXIIIA^lo, HLA-DR^hi, and CD45^hi. CD163⁺ cells were CD11c^mid, FXIIIA^hi, HLA-DR^mid, and CD45^lo. (D) A subset of BDCA-1⁺ cells was CD86^hi, CD83⁺, and DC-LAMP^hi. Representative graphs from 3 experiments. Scale bar: 100 μm.