Figure 2.

(A) At-RAB2 transcripts in poly(A)-enriched RNA from the major organs of mature plants, and dividing (3 day) and stationary phase (7 day) suspension culture cells. The lengths (in kb) and positions of the RNA molecular weight markers are indicated on the left. (B) Flower buds estimated to be greater or less than 1 mm in length were collected separately from both wild type (wt) and male sterile ms1 mutant plants. Fully open flowers were not included in the >1-mm sample. As a control, the blot was reprobed with an Arabidopsis translation initiation factor clone, eIF4A, showing that expression of this gene was similar in wild-type and ms1 mutant buds. (C) RNA was isolated from unimbibed seeds (0 day) and 2, 4, and 8 days after imbibition. The blot was reprobed with the eIF4A clone as a control.