Figure 3.

Effects of cis- and trans-acting mutations that disrupt repression by Rme1p on binding of Hap1p and Hap2p/3p/5p. In vivo UV photofootprinting of the noncoding strand was performed with intact cells (lanes C) or with purified DNA (lanes D) as in Fig. 2. UASs are shown to the left of the gels. Arrows indicate sites of enhancements of UV photoproducts in cells. Lanes 1 and 2, strain AMP1426 (α rme1::P_GAL1-S53-RME1::TRP1 gal80::LEU2 rgr1–100 sin4::TRP1) carrying plasmid pAC153–4; lanes 3 and 4, strain AMP1122 carrying plasmid pAC142–10; lanes 5 and 6, strain AMP1124 carrying plasmid pAC142–10; lanes 7 and 8, strain AMP1122 carrying plasmid pAC153ΔRS; lanes 9 and 10, AMP1124 carrying plasmid pAC153ΔRS.