Table 2. Physical and genetic locations of AFLP markers of Plasmodium chabaudi chabaudi strain AJ whose Comparative Intensities (CI) were reduced below 50% in the progeny of the genetic cross between CB-pyr10 and AJ following selection in an AJ-immunised mouse (see text).
| Names of P. c. chabaudi AJ markers | CIs of the AJ markers in AJ immunised mouse | Physical locations in the P. falciparum genome of P. falciparum orthologues of the P. c. chabaudi AJ markers [46] | P. falciparum chromosomes | Chromosome on which AJ marker is predicted to be located in P. c. chabaudi by physical mapping | Chromosome on which AJ marker is predicted to be located in P. c. chabaudi by genetic mapping | Genetic distances along the P. c. chabaudi chromosomes in centi Morgans [34] |
| AJ TG 03 AA CB | 21.7 | pf 9-378** | 9 | 8 | 8 | 22.0 |
| AJ TA 04 AT CB | 20.2 | pf 9-718 | 9 | 8 | 8 | 43.0 |
| AJ GT 02 TA CB | 13.1 | pf 9-992 | 9 | 8 | 8 | 43.0 |
| AJ AT 01 AG CB | 10.4 | pf 9-1019 | 9 | 8 | ND | ND |
| AJ TT 03 AT CB | 12.15 | pf 9-1113 | 9 | 8 | 8 | 59.4 |
| AJ TA 07 TA CB | 6.01 | pf 9-1150 | 9 | 8 | 8 | 59.4 |
| AJ TC 01 TG CB | 1.5 | pf 9-1159 | 9 | 8 | 8 | 59.4 |
| AJ allele of msp-1 | (0%, 60%)* | pf 9-1201 | 9 | 8 | 8 | 50.8 |
| AJ AG 05 AG CB | 1.5 | pf 9-1263 | 9 | 8 | 8 | 55.3 |
| AJ AT 03 AG CB | 47.5 | pf 9-1368 | 9 | 8 | ND | ND |
| AJ AC 01 CT CB | 31.8 | pf 11-1084 | 11 | 9 | 9 | 51.7 |
| AJ AG 01 TC CB | 25.9 | pf 11-1245 | 11 | 9 | 9 | 63.5 |
| AJ AT 03 TA CB | 22.7 | pf 11-1084 | 11 | 9 | 9 | 67.2 |
| AJ allele of ama-1 | (17.3%, 79.6%)* | pf 11-1290 | 11 | 9 | 9 | 71.1 |
| AJ AT 01 GT CB | 28.4 | ND | ND | ND | 7 | 37.3 |
| AJ TG 01 AT CB | 21.09 | pf 12-1259 | 12 | 14 | 7 | 77.7 |
| AJ AT 01 TC CB | 39.1 | pf 12-1267 | 12 | 14 | 7 | 77.7 |
| AJ TA 06 TA CB | 23.37 | pf 12-1290 | 12 | 14 | 7 | 77.7 |
| AJ TG 01 TC CB | 29.0 | pf 12-1346 | 12 | 14 | 7 | 77.7 |
| AJ TT 02 TG CB | 27.0 | pf 12-1355 | 12 | 14 | 7 | 81.4 |
| AJ AG 01 CA CB | 43.54 | pf 4-787 | 4 | 7 | 7 | 99.6 |
| AJ AG 02 AG CB | 27.2 | pf 14-2756 | 14 | 12 | ND | ND |
| AJ TT 01 TC CB | 26.88 | ND | ND | ND | g2 | ND |
| AJ AG 01 TA CB | 40.0 | ND | ND | ND | g12 | ND |
| AJ AT 02 TT CB | 22.22 | ND | ND | ND | g12 | ND |
| AJ TG 02 AT CB | 34.6 | ND | ND | ND | g12 | ND |
The six AJ markers with CI of <20% in the AJ-immune selected cross progeny mapped to positions closely linked to the gene encoding the P. c. chabaudi merozoite surface protein-1 (MSP-1) are indicated in bold. ND not determined.
*
The first and second numbers in brackets represent percentages of parasite DNA carrying the AJ alleles of the indicated gene ( msp-1 or ama-1), respectively in the AJ-immune selected cross progeny and in the non-immune selected cross progeny, as measured by RTQ-PCR (see text)
**
Numbers after ‘pf’’ indicate the Plasmodium falciparum chromosome number followed by distance along the chromosome in kilo base pairs