Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Aug 31;3(8):e115. doi: 10.1371/journal.ppat.0030115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 Pino et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) The predicted bipartite leader of SOD2 (SPTP) fused to reporter proteins (GFPmyc or DsRed) only localizes to the parasite mitochondrion as revealed by colocalization with the bimodally targeted SOD2 (SOD2Ty) (panels a and b), indicating that part of the information necessary for bimodal targeting of SOD2 resides in its mature protein portion. Replacement of the GFP or DsRed reporter by the predicted mature protein of SOD3 complements the bipartite leader of SOD2 (SPTP-SOD3Ty) and restores bimodal targeting to both organelles (panel c). The putative mitochondrial transit peptide of SOD3 in conjunction with the SOD2 mature protein leads to mitochondrial localization (panel d). The arrows indicate the apicoplast. Scale bars represent 2 μm.

(B) Western blot analysis of parasites stably expressing SOD2Ty, SOD3Ty, SPTP-SOD3Ty, and TP(SOD3)-SOD2Ty using anti-Ty-1 antibodies.

(C) Western blot analysis of parasite clones stably expressing SOD2myc and SPTP(SOD2)-GFPmyc using anti-myc and anti-actin antibodies, showing that the bimodal targeting of SOD2myc is not due to a difference in the expression level compared with the mitochondrion-targeted SPTP-GFPmyc.