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. 2007 Aug 31;3(8):e115. doi: 10.1371/journal.ppat.0030115

Figure 7. Bimodal Targeting of T. gondii ACN/IRP.

Figure 7

(A) Alignment of TgIRP/ACN with related protein sequences. Red arrowheads indicate theoretical alternative start-methionines. A putative SP as predicted by SignalP is underlined in black, and putative mitochondrial transit peptides (as predicted by TargetP or MitoProt) are underlined in red (both for the full-length sequence and for a hypothetical protein starting from the second methionine at position 74).

(B) Colocalisation reveals bimodal targeting of SPTP(ACN)GFP-Ty fusion protein to the parasite mitochondrion and apicoplast. A deletion of the SP targets the fusion protein (TP(ACN)-GFPTy) to the mitochondrion only, while a mutation of the second methionine in position 74 within the TP results in bimodal targeting (SPTP(ACN)-M74I-GFP-Ty). The asterisk indicates the position of the mutation M74I. The arrows indicate the apicoplast. Scale bars represent 2 μm.

(C) Colocalization of TgICDH1Ty (left panel) with the apicoplast marker FNRDsRed (middle panel) indicates a localization of ICDH1 around the periphery of the plastid organelle. Scale bars represent 2 μm.