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Products of in vitro translation remain associated with ribosomes. After incubation, translation mixtures were applied to sucrose gradients and centrifuged. The absorbance at 254 nm (dotted line) and the 35S in trichloroacetic acid precipitates of portions of the individual fractions (•) are shown. Translation mixtures contained (A) mRNA for p370 and (B) mRNA for m370.
