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. Author manuscript; available in PMC: 2007 Aug 30.
Published in final edited form as: J Biol Chem. 2002 Aug 23;277(44):41539–41546. doi: 10.1074/jbc.M204850200

Fig. 4. The cytoplasmic localization of HuR is regulated by amino acid availability.

Fig. 4

A, C6 cells were grown on chamber slides and were either incubated in amino acid-depleted medium (Starved) for 4 h or heat shocked at 45 °C for 2 h. Nuclei are marked by arrows. The margin of one starved cell is marked by arrowheads. B, cells were incubated for the indicated times in either amino acid-containing (Control) or amino acid-depleted medium (S). Cells were also treated with actinomycin D (1 μg/ml) for 4 h. One sample was starved for 6 h followed by 3 h incubation in medium containing amino acids (S6h/F3h). Cells were fixed, permeabilized, and prepared for confocal microscopy (A) or immunofluorescence microscopy (B) using an anti-HuR antibody, as described under “Experimental Procedures.”