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. 2007 Aug 9;104(34):13690–13695. doi: 10.1073/pnas.0705053104

Fig. 1.

Fig. 1.

BRD4 interacts with P-TEFb by means of a short CTD. (A) FLAG-tagged deletion mutants of BRD4 were overexpressed in 293T cells. Nuclear extracts (NE) and anti-FLAG immunoprecipitates (IP) were immunoblotted (IB) for CDK9 and cyclin T1. The small arrows point to the full-length proteins, and the smaller bands likely reflect degradation products. (B) FLAG-tagged BRD4 constructs encoding residues 1209–1362, 1209–1362Δ1279–1304, 1209–1362Δ1306–1330, 1209–1362Δ1329–1362, 1209–1362Δ1329–1345, and 1209–1362Δ1345–1362 were overexpressed in 293T cells. Anti-FLAG immunoprecipitates were immunoblotted with anti-CDK9 or anti-cyclin T1.