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Histology from pemphigus patients whose sera were used for this study revealed typical blister localization (a and b). The H&E-stained paraffin sections showed suprabasal epidermal cleavage in the PV histology and superficial blistering in the PF lesion. In human skin in vitro (c and d), PV-IgG-induced splitting was suprabasally located, whereas PF-IgG-induced cleavage was found within the spinous layer. Bar = 50 μm in all panels.
