Table 2.
Temporal Cortex SI Proteins Identified in iTRAQ Experiment
| Protein name | Peptide count | Total ion score C.I. % | Previously ID’ed as SI in AD41 |
|---|---|---|---|
| 14-3-3 protein sigma* | 1 | 99.89 | + |
| 17-kd protein | 1 | 95.24 | |
| 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase, isoform CNPII | 3 | 100.00 | + |
| 20-kd protein | 1 | 100.00 | |
| 33-kd protein | 1 | 96.70 | |
| 365-kd protein | 5 | 100.00 | |
| 40S ribosomal protein S30 | 1 | 100.00 | |
| 5′-3′ exoribonuclease 2 | 1 | 98.67 | |
| 60S ribosomal protein L7a | 1 | 100.00 | |
| 62-kd protein | 1 | 99.88 | |
| Actin, cytoplasmic 2* | 5 | 100.00 | + |
| ADP/ATP translocase 1 | 3 | 100.00 | |
| ADP/ATP translocase 2 | 3 | 100.00 | |
| Aldehyde dehydrogenase, mitochondrial | 1 | 97.69 | |
| α Crystallin B chain | 2 | 100.00 | + |
| α-Synuclein, isoform 2-4 | 1 | 99.85 | |
| Amyloid β A4* | 1 | 100.00 | + |
| Apolipoprotein D | 2 | 100.00 | + |
| Apolipoprotein E* | 8 | 100.00 | + |
| ATP synthase e chain, mitochondrial | 1 | 96.68 | |
| ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2 isoform 2a | 1 | 99.10 | |
| Brain acid-soluble protein 1 | 1 | 100.00 | |
| Calcium/calmodulin-dependent protein kinase type II α chain | 6 | 100.00 | + |
| Carbonyl reductase 1 | 1 | 96.81 | + |
| Cathepsin D | 4 | 100.00 | + |
| CDNA FLJ44161 fis, clone THYMU2033070 | 1 | 97.34 | |
| Clusterin isoform 1 | 1 | 99.86 | |
| Cofilin-2, isoform CFL2b | 1 | 99.53 | + |
| Collagen α-1(I) chain | 5 | 100.00 | |
| Collagen α-1(III) chain | 1 | 99.98 | |
| Collagen α-2 type IV* | 4 | 100.00 | + |
| Collagen α-2(I) chain | 4 | 100.00 | + |
| Collagen, type XXII, α-1 | 1 | 99.30 | |
| Contactin-1, isoform 2 | 1 | 99.37 | |
| Creatine kinase B-type | 3 | 100.00 | + |
| Creatine kinase, ubiquitous mitochondrial | 4 | 100.00 | + |
| Cytochrome c oxidase polypeptide Vb | 1 | 99.98 | + |
| Cytochrome c oxidase polypeptide VIc | 1 | 99.81 | + |
| Cytochrome c oxidase polypeptide VIIa | 1 | 99.92 | + |
| Cytochrome c oxidase subunit 4 isoform 1 | 3 | 100.00 | + |
| Desmuslin, isoform 2 | 1 | 98.71 | |
| Disheveled-associated activator of morphogenesis 2 | 1 | 97.80 | |
| F-box-like/WD-repeat protein TBL1XR1 | 1 | 96.01 | |
| Ferritin heavy chain | 3 | 100.00 | + |
| Fibrinogen β chain | 1 | 98.02 | + |
| FSCN1 protein | 1 | 99.98 | |
| FTL protein | 1 | 98.91 | |
| Glial fibrillary acidic protein* | 3 | 100.00 | + |
| Glutamate transporter variant EAAT1ex9skip | 1 | 96.16 | |
| Glyceraldehyde-3-phosphate dehydrogenase* | 2 | 100.00 | + |
| Guanine nucleotide-binding protein α-12 subunit | 1 | 100.00 | + |
| Guanine nucleotide-binding protein G(o), α subunit 2 | 2 | 100.00 | |
| HANP1 | 1 | 96.50 | |
| Heat shock-related 70 kd* | 2 | 100.00 | + |
| Heterogeneous nuclear ribonucleoprotein K, isoform 1 | 1 | 99.56 | |
| Heterogeneous nuclear ribonucleoprotein Q, isoform 4 | 1 | 98.60 | |
| Heterogeneous nuclear ribonucleoproteins A2/B1, isoform A2 | 3 | 100.00 | |
| Histone H1.0 | 3 | 100.00 | + |
| Histone H1.3 | 2 | 100.00 | + |
| Histone H2A type 3 | 2 | 100.00 | + |
| Histone H2B* | 4 | 100.00 | + |
| Histone H3.4 | 2 | 100.00 | + |
| Histone H4 | 3 | 100.00 | + |
| Tubulointerstitial nephritis antigen-like, isoform 1 | 4 | 100.00 | + |
| Cleavage and polyadenylation specificity factor 6, isoform 6 | 1 | 98.27 | |
| Laminin α-5 chain | 2 | 100.00 | |
| Laminin β-2 chain | 4 | 100.00 | |
| Laminin γ-1 chain | 2 | 100.00 | + |
| Methylcrotonoyl-CoA carboxylase β chain, isoform 1 | 2 | 100.00 | |
| Microtubule-associated protein tau, isoform A* | 4 | 100.00 | + |
| Microtubule-associated protein tau, isoform B* | 4 | 100.00 | + |
| Myelin basic protein* | 4 | 100.00 | + |
| NG22 protein isoform 1 | 1 | 96.03 | |
| Nidogen-2 | 1 | 99.98 | + |
| P2X7 isoform D | 1 | 98.24 | |
| Palmitoyl-protein thioesterase 1 | 1 | 100.00 | + |
| Pleckstrin homology-like domain family A member 3 | 1 | 97.99 | |
| Prion protein | 1 | 100.00 | |
| Prosaposin | 3 | 100.00 | |
| Proteasome β4 subunit | 1 | 100.00 | |
| Proteasome subunit α type 1 | 1 | 100.00 | |
| Proteasome subunit β type 2 | 1 | 99.00 | |
| Proteasome subunit β type 6 | 1 | 99.98 | |
| Protein KIAA0157 | 1 | 98.41 | |
| Proteolipid protein 1 isoform 1 | 1 | 100.00 | + |
| Ras-associated and pleckstrin homology domain-containing | 1 | 98.04 | |
| RNA binding motif protein, X-linked-like 1 | 1 | 98.80 | |
| Serine/threonine-protein kinase 38-like | 1 | 96.39 | |
| Serine/threonine-protein kinase RIO2 | 1 | 98.06 | |
| Similar to elongation factor Tu GTP binding domain | 1 | 99.18 | |
| Splicing factor 3A subunit 2 | 1 | 99.85 | |
| Synapsin-1, isoform IA | 5 | 100.00 | + |
| Syntaxin-binding protein 1, isoform 1 | 1 | 99.98 | + |
| Tripeptidyl-peptidase 1 precursor, isoform 3 | 1 | 99.99 | + |
| Tubulin α-1 chain | 3 | 100.00 | |
| Tubulin α-3 chain | 5 | 100.00 | |
| Tubulin α-ubiquitous chain | 5 | 100.00 | + |
| Tubulin β-2C chain* | 7 | 100.00 | |
| Tubulin, β polypeptide* | 6 | 100.00 | + |
| Tubulin, β, 4* | 5 | 100.00 | + |
| Ubiquitin* | 4 | 100.00 | + |
| Up-regulated in colorectal cancer gene 1 protein | 1 | 96.08 | |
| USP36 protein | 1 | 95.69 | |
| Vitamin K-dependent protein Z precursor | 1 | 96.15 | |
| Wolf-Hirschhorn syndrome candidate 1 protein isoform 4 | 1 | 98.26 | |
| Zinc finger, CW type with coiled-coil domain 2 | 1 | 97.07 |
C.I., confidence interval; CoA, coenzyme A.
*
SI proteins validated by Western blot in our previous study.41