Figure 6.

Effect of p65 and c-Rel transfection on the NOS-2 promoter activity. Primary cultures of macrophages (Mφ), neonatal (NeoC), and the adult cardiomyocytic cell line HL-1 were cotransfected with the indicated vectors, and the luciferase activity was determined after 24 hours of culture. Results are expressed as the luminescence ratio between the light corresponding to the reporter activity versus the light of Renilla luciferase used as normalization vector. Data show the mean ± SD of four experiments. *P < 0.01 versus the control condition; ^#P < 0.01 versus the condition in the absence of p65 and c-Rel proteins.