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. 2007 Jul 16;7:41. doi: 10.1186/1472-6750-7-41

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Copyright © 2007 Pluta et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Schematic representation of lentiviral vectors containing modified U6 promoter sequences or Tet-dependent repressor and transactivator sequences. (A) Promoters. DSE, distal sequence element; PSE, proximal sequence element; TO, tetracycline operator; TATA, TATA box; hU6, human U6 promoter; Tet-hU6, human U6 promoter with an internal TO; TRE/TetU6, a second–generation TRE, lacking a TATA box and consisting of eight repositioned TO sequences placed upstream of the Tet-hU6 promoter; PittΔU6, hU6 promoter with DSE element deleted and replaced using a second-generation TRE lacking a TATA box. (B) Repressors and transactivators. P_CMV, human cytomegalovirus immediate early promoter; TetR, tetracycline repressor; KRABAB, KRAB-AB domain of the Kid-1 protein; rtTA, amino acids 1–207 derived from the Tet-controlled rtTA2^S-M2 reverse transactivator; Sp1-AB, Sp1-B and Sp1-pB, Sp1-derived transactivation domains.