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. 2007 May 11;93(6):2091–2101. doi: 10.1529/biophysj.106.096479

FIGURE 4.

FIGURE 4

Fluorescence lifetime image of a muscle fiber incubated with 10 μM 3′-O-DEAC-pda-ATP. (A) Intensity image. (B) FLIM image, where the fluorescence decay for each pixel was fit to a single exponential decay. The color represents the decay lifetime according to the pseudocolor scale on the right side of the image. The I-band has an average lifetime of 1.98 ± 0.02 ns (mean ± SD, n = 13 pixels), and the A-band has an average lifetime of 2.07 ± 0.03 ns (n = 34 pixels). (C) Lifetime (blue line) and intensity (green line) profiles averaged over one sarcomere but displayed for two sarcomeres. The fiber has a sarcomere length (SL) of 2.41 μm (myosin filament overlap 90% with actin). A, A-band; I, I-band; scale bar, 2 μm.