TABLE 1.

Sedimentation equilibrium studies of IL-8 7–34 and 9–50 disulfide mutants

Protein	Buffer	Kd (μM)*
WT IL-8	50 mM phosphate, pH 7	12 ± 5
	50 mM acetate, pH 5.2	6 ± 3
7–34 disulfide mutant	50 mM phosphate, pH 7	53 ± 12
	50 mM acetate, pH 5.2	19 ± 9
9–50 disulfide mutant	50 mM phosphate, pH 7	58 ± 9
	50 mM acetate, pH 5.2	24 ± 4

^*The K_d reported for data collected at rotor speed of 30,000 rpm. Similar values were obtained at 25,000 and 35,000 rpm, and global fit of the data at all three speeds did not significantly improve the fit. The phosphate buffer also contains 50 mM NaCl