TABLE 2.

Equilibrium unfolding studies of IL-8 7–34 and 9–50 disulfide mutants

Protein	Buffer	ΔG (kcal/mol)	C½ (M)
Native IL-8	50 mM phosphate, pH 7.0	−6.0 ± 0.1	4.3 ± 0.1
	50 mM acetate, pH 5.2	−7.0 ± 0.1	3.9 ± 0.1
7–34 disulfide mutant	50 mM phosphate, pH 7.0	−3.2 ± 0.5	3.4 ± 0.1
	50 mM acetate, pH 5.2	−2.7 ± 0.7	3.0 ± 0.2
9–50 disulfide mutant	50 mM phosphate, pH 7.0	−2.2 ± 0.5	1.5 ± 0.2
	50 mM acetate, pH 5.2	−2.1 ± 0.2	1.5 ± 0.1