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. Author manuscript; available in PMC: 2008 Jun 20.
Published in final edited form as: Virology. 2007 Feb 16;363(1):36–47. doi: 10.1016/j.virol.2007.01.010

Figure 5.

Figure 5

Intracellular cytokine flow cytometry for IFN-γ and TNF-α responses from a representative SHIVsf162p3 infected macaque (L274). PBMC were unstimulated (medium control), or stimulated for 6 h with different peptide pools at day 56 post inoculation. Cells were gated first on lymphocytes followed by CD3+ and then on CD8+ T cells. The percentages of IFN-γ and/or TNF-α positive cells are shown in each quadrant. Bold numbers represent positive responses. Four color CFC staining was performed to define antigen specific cytokine responses as described in Table 1.