Table 2.
Summary of SCID mice T and V tumor cell line injection experiments
| Cell lines
|
Metastatic assay
|
Sc. injections
|
||||
|---|---|---|---|---|---|---|
| No. of lines injected
|
No. mice injected
|
Macroscopic tumors >20% lungs
|
Microscopic tumors 50–250 μ dia.
|
Time of death after injection
|
Time to form tumors to 2 cm size
|
|
| V p18-20 | 2 | 8 | 5/8 | 2/8 | >12 wk | 5–6 wk |
| V p98-110 | 3 | 12 | 1/12* | 5/12 | >12 wk | 5–6 wk |
| V p300 | 3 | 12 | 0/12 | 7/12 | >12 wk | 5–7 wk |
| V p18-20 + mTERC | 3 | 12 | 8/12 | n.a. | 1.5–3 wk | 4–6 wk |
| V p98-110 + mTERC | 3 | 21 | 17/21 | 2/21 | 1.5–3 wk | 4–6 wk |
| V p305-310 + mTERC | 2 | 8 | 8/12 | 1/12 | 1.5–3 wk | 4–6 wk |
| V p18-21 + vector | 2 | 4 | 0/4 | 3/4 | >12 wk | 5–7 wk |
| V p98-110 + vector | 3 | 8 | 0/8 | 3/8 | >12 wk | 5–7 wk |
| V p305-310 + vector | 3 | 9 | 0/9 | 2/9 | >12 wk | 6–8 wk |
| SCID V p6 | 1 | 4 | 0/4 | 2/4 | >12 wk | 6–12 wk |
| SCID V p108-110 | 2 | 9 | 0/9 | 5/9 | >12 wk | 7–11 wk |
| SCID-1 V p300 | 2 | 12 | 0/12 | 6/12 | >12 wk | 4–8 wk |
| SCID-2 V p300 | 5 | 15 | 0/15 | 8/15 | sac at 7 wk | 1–2 wk |
| SCID-2V p300 + mTERC | 5 | 15 | 15/15 | n.a. | 5 wk | n.d. |
| T p21-25 | 3 | 7 | 6/7 | 0/7 | 5–6 wk | 4.5 wk |
| T p125 | 1 | 4 | 4/4 | n.a. | 4–5 wk | 4–4.5 wk |
| T p300 | 3 | 12 | 10/12 | 2/12 | 4–5 wk | 4–5 wk |
| SCID T p23 | 1 | 6 | 6/6 | n.a. | 4–5 wk | 4–5 wk |
| SCID T p125 | 1 | 7 | 2/7 | 2/7 | 5–6 wk | 5–6 wk |
| SCID-1 T p300 | 2 | 8 | 6/8 | 1/8 | 4–5 wk | 4–5 wk |
| SCID-2 T p300 | 3 | 12 | 8/12 | 2/12 | 4–5 wk | 4–5 wk |
| G0 mTERC+/− | 2 | 4 | 4/4 | n.a. | 3–4 wk | 4–6 wk |
| G2 mTERC−/− | 2 | 4 | 4/4 | n.a. | 3–4 wk | 4–6 wk |
*
This lesion was 300 μ in diameter.
n.d. indicates not done; n.a. indicates not applicable (macroscopic tumors obliterated lung field).