Skip to main content
. 2003 Jan 1;17(1):31–36. doi: 10.1101/gad.1041203

Figure 1.

Figure 1

Dependence of Vg1 and GDF1 signaling on Oep but not on Nodal signals. (A–F) Live zebrafish embryos at 24 hpf, anterior to the left, dorsal up. (G–U) ntl mRNA expression in zebrafish embryos at shield stage, animal pole view; dorsal to the right in H, I, J, K, L, N, O, and Q. (A,G) LacZ control-injected wild type. (B,H) LacZ control-injected MZoep. (A,C,E) Wild-type embryos. (G,J,M,P,S) Wild-type heterozygous siblings (cycm294/+;sqcz35/+ from a cycm294/+; sqtcz35/+ intercross). (B,D,F,H,K,N,Q,T) MZoep embryos. (I,L,O,R,U) cyc;sqt double mutant embryos. Embryos were injected with 250 pg of LacZ mRNA (A,B,G,H,I); 100 pg (C,J,K,L) or 250 pg (D) of aVg1 mRNA; 100 pg (E) or 250 pg (F,M,N,O) of bGDF1 mRNA; 20 pg of squint mRNA (P,Q,R); and 20 pg (T) or 100 pg (S,U) of activin mRNA. (G) Note the wild-type expression of ntl in marginal cells. (H) In MZoep mutants, ntl expression is absent dorsally at the margin. Wild-type embryos are severely dorsalized or have ectopic ntl expression when injected with aVg1 (C,J), bGDF1 (E,M), squint (P), and activin (S) mRNA. In contrast, MZoep embryos injected with aVg1 (D,K), bGDF1 (F,N), or squint (Q) mRNA are indistinguishable from the LacZ control-injected embryos. Similar to wild-type embryos, expression of ntl is expanded in cyc;sqt double mutants injected with aVg1 (L), bGDF1 (O), squint (R), or activin (U) mRNA.