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. 1997 Feb 4;94(3):820–825. doi: 10.1073/pnas.94.3.820

Figure 1.

Figure 1

The yeast YDR1 gene encodes the homologue of the human Dr1 repressor. (A) Sequence alignment of the human (hDr1) and yeast (yDr1) proteins. Identical residues are denoted by |; similar residues are denoted by : or .. A single gap (… ) was introduced into the Ydr1 sequence to maintain the alignment. (B) YDR1 is essential for cell viability. The YDR1 coding region (open rectangle) was replaced by the HIS3 gene and the resulting construct was used to disrupt a single copy of YDR1 in a his3−/his3− YDR1/YDR1 diploid strain. Sporulation and dissection yielded 2 viable spores for each of 18 tetrads, all of which are phenotypically His−. (C) Expression of human Dr1 restores viability to a ydr1::HIS3 null mutant. Plasmid shuffle strain YMH196 (ydr1::HIS3 leu2 [YDR1-URA3]), carrying either the MET25-LEU2 plasmid p415 (vector), or its derivatives expressing YDR1 behind its own promoter (YDR1), or human (MET25/hDr1), or yeast (MET25/yDr1) Dr1 behind the MET25 promoter, were cured of YDR1-URA3 under conditions that induce the MET25 promoter (-Met/5-FOA).