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. 2003 Jan 1;17(1):49–63. doi: 10.1101/gad.1048103

Figure 7.

Figure 7

An endogenous wheat nuclease efficiently cleaves wild-type but not mutant PHV target RNAs. (A) When incubated in wheat germ extract, 5′-radiolabeled target RNA containing wild-type PHV sequences was cleaved within the PHV sequences complementary to miR165 and miR166. In contrast, a dominant G → A mutant target RNA was cleaved inefficiently. (B) Quantification of the data in A. (Circles) Wild-type PHV sequences; (squares) mutant sequences; (filled symbols) full-length target RNA; (open symbols) 5′ cleavage product. The difference in cleavage rates is ∼14-fold. (C) Analysis of PHV cleavage in an in vitro RNAi reaction programmed with siRNA duplexes and Drosophila embryo lysate. The identity of the antisense strand of the siRNA duplex and the RNA target used is indicated above the gel and described in Figure 6C.