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. 2003 Feb 1;17(3):359–367. doi: 10.1101/gad.1047003

Figure 3.

Figure 3

Western blot analysis showing CIAP1 cleavage is associated with p53 induction and is caspase- and apoptosis-independent. Cell lysates from HeLa-CIAP1 with different treatments (as indicated on top: Fas-Ab, treatment of 1 μg/mL Fas antibody for 24 h; Etop, treatment of 10 μM etoposide for 24 h; z-VAD, treatment of 100 μg/mL z-VAD for 25 h; z-VAD + ETOP, treatment of 100 μg/mL z-VAD for 1 h prior to additional 10 μM etoposide treatment another 24 h) were analyzed by immunoblot assay with different antibodies (as indicated on the left side). The identities of each band are indicated to the right. The results are representative data from three independent experiments.